NYREN18 Colorimetric Cell-Based ELISA Kit

Assay Genie's NYREN18 Colorimetric Cell-Based ELISA Kit is a lysate-free, high-throughput assay designed to detect the NYREN18 protein (also known as NUB1) in cultured cells using an indirect ELISA format. The kit is reactive with human and mouse samples and uses a colorimetric detection method read at 450 nm, with a dynamic range of > 5000 cells. It is supplied in a 96-well microplate format and is intended for use with adherent cells.

The assay captures NYREN18 via target-specific primary antibodies, with HRP-conjugated secondary antibodies binding the Fc region of the primary antibody to catalyse a colorimetric reaction upon substrate addition. A monoclonal antibody specific for human GAPDH is included as an internal positive control for normalising target absorbance values, and a Crystal Violet whole-cell staining method may be used to determine cell density and adjust for plating differences. The kit is suitable for measuring relative NYREN18 levels in cultured cells and for screening the effects of treatments, inhibitors (such as siRNA or chemicals), or activators on NYREN18 expression.

Storage is at 4 °C with a stability of 6 months. According to the supplied database information, NYREN18 (UniProt ID: Q9Y5A7, Gene ID: 51667) functions as a specific down-regulator of the NEDD8 conjugation system, recruiting NEDD8, UBD, and their conjugates to the proteasome for degradation, and has a molecular weight of 69,119 Da.