NRG1 Isoform-10 Colorimetric Cell-Based ELISA Kit

Assay Genie offers a Cell-Based ELISA Kit designed for the qualitative determination of NRG1 isoform-10 protein expression in cultured cells. The assay uses an indirect ELISA format in which the target is captured by NRG1 isoform-10-specific primary antibodies, and HRP-conjugated secondary antibodies bind the Fc region of the primary antibody, catalysing a colorimetric reaction upon substrate addition. Described as lysate-free and high-throughput, the kit can be used to measure relative NRG1 isoform-10 levels and to screen the effects of treatments, inhibitors (such as siRNA or chemicals) and activators.

A monoclonal antibody specific for human GAPDH is included to serve as an internal positive control for normalising target absorbance values, and a Crystal Violet whole-cell staining method is provided to determine cell density after the colorimetric measurement. According to the manufacturer, the kit is intended for adherent cells, with a detection wavelength of 450 nm, a microplate format, storage at 4 °C for 6 months, a dynamic range of greater than 5000 cells, and reactivity with human, mouse and rat samples.

NRG1 isoform-10 is a membrane glycoprotein that acts as a direct ligand for ERBB3 and ERBB4 tyrosine kinase receptors. According to the manufacturer, isoform 10 is expressed in the nervous system, including spinal cord motor neurons, dorsal root ganglion neurons and brain, and is the predominant isoform in sensory and motor neurons, with dysregulation of NRG1 linked to conditions such as schizophrenia and bipolar disorder. The product is associated with Gene ID 3084 and UniProt ID Q02297-10.