NR2F6 Transcription Factor Activity Assay

Assay Genie provides this kit to quantify functional DNA-binding activity of NR2F6, an orphan nuclear receptor also known as EAR-2 that regulates gene programs linked to T-cell checkpoint control and tumor immune evasion. The workflow uses an oligonucleotide-coated plate presenting the AGGTCA-like response element recognized by NR2F6, capturing the active transcription factor from prepared nuclear and cell lysates before target-specific antibody-based detection. Researchers employ the microplate workflow to compare activation states across human, mouse, and rat samples, supporting studies in immuno-oncology, metabolic disease, and stem cell biology. The kit is intended for research use only in cell culture and in vitro models, supporting medium-throughput screening of transcription factor binding across experimental conditions.