nNOS (Phospho-Ser852)Colorimetric Cell-Based ELISA Kit

From Assay Genie, this cell-based ELISA kit is designed for the accurate detection of phosphorylated nNOS (neuronal nitric oxide synthase) at Ser852 in cell lysates and tissue homogenates. It uses an indirect colorimetric ELISA format read at 450 nm, with HRP-conjugated secondary antibodies catalyzing a color reaction upon substrate addition. The assay is lysate-free, high-throughput and sensitive, enabling measurement of relative phosphorylated nNOS levels in cultured cells as well as screening for the effects of treatments, inhibitors (such as siRNA or chemicals) or activators on nNOS phosphorylation.

The kit is reactive with Human, Mouse and Rat samples and has a dynamic range of > 5000 cells. Because of the qualitative nature of the cell-based ELISA, two normalization methods are built in: a monoclonal anti-human GAPDH antibody serves as an internal positive control for target absorbance values, while Crystal Violet whole-cell staining can be used to determine cell density and adjust for plating differences. The gene symbol is NOS1 (UniProt P29475).

Phosphorylation of nNOS at Ser852 is associated with regulation of its enzymatic activity, making this marker relevant to studies of signaling pathways, drug development and potential therapeutic interventions in areas such as neurodegenerative disorders, cardiovascular disease and inflammation.