nNOS Colorimetric Cell-Based ELISA Kit

Assay Genie offers a cell-based ELISA kit for detecting and quantifying nNOS (Neuronal Nitric Oxide Synthase) protein expression directly in cultured cells, including adherent cell samples, without the need for lysate preparation. The qualitative, high-throughput indirect immunoassay captures the target using nNOS-specific primary antibodies, while HRP-conjugated secondary antibodies bind the Fc region of the primary antibody; the conjugated HRP enzyme then catalyses a colorimetric reaction measured at 450 nm upon substrate addition.

The kit is suited to measuring relative nNOS levels in cultured cells and to screening the influence of inhibitors (such as siRNA or chemicals), activators or other treatments on nNOS expression. A monoclonal antibody specific for human GAPDH is included as an internal positive control for normalising target signals, and a Crystal Violet whole-cell staining step is provided to determine cell density so that absorbance values can be normalised to cell number, adjusting for plating differences.

Reactivity has been validated for human, mouse and rat samples, with a stated dynamic range of > 5000 cells. Storage is at 4 °C with a stated stability of 6 months. nNOS (gene symbol NOS1, UniProt P29475, Gene ID 4842) is a key enzyme that produces nitric oxide, a messenger molecule in the nervous system whose dysregulation has been linked to neurological disorders including Alzheimer's disease, Parkinson's disease and stroke.