NMDAR2B (Phospho-Tyr1474)Colorimetric Cell-Based ELISA Kit

From Assay Genie, this cell-based ELISA kit enables the qualitative measurement of phosphorylated NMDAR2B (Phospho-Tyr1474) in cultured cells. Designed for adherent cell samples, the kit uses a colorimetric detection method at 450 nm in an indirect ELISA format: phospho-specific primary antibodies capture the target, and HRP-conjugated secondary antibodies catalyse a colour reaction upon substrate addition. It is lysate-free, sensitive and suited to high-throughput workflows, with a stated dynamic range of > 5000 cells. The assay can be used to compare relative NMDAR2B phosphorylation across treatments — including inhibitors such as siRNA or chemical compounds, and activators — with reactivity validated for human, mouse and rat samples.

For normalisation, a monoclonal anti-GAPDH antibody is included as an internal positive control, and a Crystal Violet whole-cell staining step allows absorbance values to be corrected for cell density to compensate for plating differences. The kit is intended for research into NMDAR2B signalling at tyrosine 1474, a recognised regulatory event in synaptic transmission and plasticity, and of interest in neurological disorders such as Alzheimer's disease, schizophrenia and epilepsy.