NM23 Colorimetric Cell-Based ELISA Kit

From Assay Genie, this Cell-Based ELISA Kit is designed for the qualitative detection of NM23 protein expression directly in cultured cells, eliminating the need for lysate preparation. The assay uses an indirect ELISA format in which NM23 is captured by target-specific primary antibodies, with HRP-conjugated secondary antibodies binding the Fc region of the primary antibody to catalyze a colorimetric reaction upon substrate addition. Detection is read at 450 nm, and a monoclonal antibody specific for human GAPDH is included to serve as an internal positive control for normalizing target absorbance values.

The kit is reactive with Human, Mouse, and Rat samples and is intended for use with adherent cells, with a stated dynamic range of > 5000 Cells. It can be used to measure relative NM23 levels in cultured cells and to screen the effects of treatments, inhibitors (such as siRNA or chemicals), or activators on NM23 expression. Following the colorimetric measurement, a Crystal Violet whole-cell staining method may be applied to determine cell density, allowing absorbance values to be normalized to cell amounts and correcting for plating differences. The product is formatted as a 96-Well Microplate with recommended storage at 4°C for 6 months stability, and is categorised within the Metabolism research area.