NF-kappaB p65 (Phospho-Thr435)Colorimetric Cell-Based ELISA Kit

This Cell-Based ELISA Kit from Assay Genie is designed for the accurate detection of phosphorylated NF-kappaB p65 at Thr435 in cell lysates, supporting research into NF-kappaB signaling pathways. The assay is lysate-free and uses a colorimetric detection method at 450 nm, providing a high-throughput and sensitive approach to measuring the relative levels of phosphorylated NF-kB p65 in cultured cells.

The kit works in an indirect ELISA format. Target phosphorylation is captured using NF-kappaB p65 (Phospho-Thr435)-specific primary antibodies, and HRP-conjugated secondary antibodies bind the Fc region of the primary antibody to catalyze a colorimetric reaction upon substrate addition. A monoclonal antibody specific for human GAPDH is included as an internal positive control for normalization of target absorbance values, and a Crystal Violet whole-cell staining method may be used to determine cell density and adjust for plating differences. The kit's stated dynamic range is > 5000 Cells.

Reactive with Human, Mouse, and Rat samples, the kit is intended for use with adherent cells. The target is the RELA gene product (Gene ID: 5970; UniProt ID: Q04206), a transcription factor involved in inflammatory and immune responses.