NF-kappaB p65 (Phospho-Ser529)Transcription Factor Activity Assay

Assay Genie offers a colorimetric ELISA kit for the detection and qualitative analysis of active NF-kappaB p65 (Phospho-Ser529) transcription factors in eukaryotic nuclear or cell lysates. The listed detection method is “450 nm,” and the stated assay time is “4.5 hours.” It is for research use only.

Listed reactivity covers human, mouse, and rat, with nuclear or cell lysates listed as the sample types. The analyte is NF-kappaB p65 phosphorylated at serine 529; “Nuclear factor NF-kappa-B p65 subunit” is listed as a synonym for the target.

Storage instructions specify “4°C for 6 months.” The manufacturer describes the assay as a tool for studying NF-kappaB p65 activity and activation status, noting the protein’s role in regulating gene expression related to inflammation, immunity, and cell survival.