NF-kappaB p65 (Acetyl-Lys310)Fluorometric Cell-Based ELISA Kit

From Assay Genie, this fluorometric cell-based ELISA kit is designed to monitor NF-kappaB p65 (Acetyl-Lys310) protein phosphorylation and expression profiles in cells. The qualitative assay uses an indirect ELISA format in which target-specific primary antibodies are detected by Dye 1- and Dye 2-conjugated secondary antibodies, allowing a fluorometric readout at Ex/Em 651/667 (Dye 1) and 495/521 (Dye 2). Two normalization strategies are built in: a monoclonal antibody specific for human GAPDH serves as an internal positive control for normalizing target RFU values, and an antibody against the non-phosphorylated counterpart of NF-kappaB p65 (Acetyl-Lys310) is provided so that RFU values for non-phosphorylated NF-kappaB p65 can be used to normalize the RFU value for the phosphorylated target.

Reactivity is listed for Human and Mouse samples, with storage at 4°C and a stability of 6 Months. The dynamic range supports measurements exceeding 5000 cells. The target is designated Phospho-RELA (Lys310), with the NCBI Gene Symbol given as RELA, and associated identifiers including Gene ID: 5970, UniProt ID: Q04206, OMIM #: 164014, and Unigene #: Hs.502875.