NF-kappaB p105/p50 (Phospho-Ser337)Colorimetric Cell-Based ELISA Kit

From Assay Genie, this Cell-Based ELISA kit is designed for the qualitative detection of phosphorylated NF-kappaB p105/p50 at Ser337 in adherent cells. The assay uses a colorimetric readout at 450 nm in an indirect ELISA format, in which NF-kappaB p105/p50 (Phospho-Ser337) is captured by target-specific primary antibodies and detected using HRP-conjugated secondary antibodies that catalyze a colorimetric reaction upon substrate addition.

The kit provides a lysate-free, high-throughput and sensitive method for measuring the relative amounts of phosphorylated NF-kappaB p105/p50 in cultured cells, and for screening the effects of various treatments, inhibitors (such as siRNA or chemicals), or activators on NF-kappaB p105/p50 phosphorylation. Reactivity is with human samples. A monoclonal antibody specific for human GAPDH is included to serve as an internal positive control in normalizing target absorbance values, and a Crystal Violet whole-cell staining method may be used to determine cell density, so that absorbance values can be normalized to cell amounts.

NF-kappaB is a key transcription factor involved in regulating immune and inflammatory responses, cell proliferation, and survival, and phosphorylation of p105/p50 at serine 337 plays a critical role in its activation and translocation to the nucleus to initiate gene expression. The dynamic range is > 5000 Cells. Associated database identifiers for the target are Gene ID 4790, UniProt ID P19838, OMIM 164011, and Unigene Hs.654408, with gene symbol NFKB1.