NF-kappaB p100/p52 Transcription Factor Activity Assay

The NF-κB p100/p52 DNA-binding ELISA from Assay Genie measures the DNA-binding activity of the p100 and p52 transcription factor subunits in nuclear extracts, supporting research into the non-canonical NF-κB signaling cascade. Supplied as a 96 Assays kit, the workflow captures active p100/p52 complexes onto plate-bound oligonucleotides containing an NF-κB consensus sequence, followed by subunit-specific antibody detection and a chromogenic substrate for absorbance-based signal development. Validated for nuclear and cell lysates from human, mouse, and rat samples, the assay is applied in studies of B-cell maturation, lymphotoxin and CD40-driven signaling, inflammation models, and tumor biology where processing of p100 to p52 is a key regulatory step. The format is intended for research use only and provides a quantitative read-out of pathway activation rather than total protein abundance.