NEK9 (Phospho-Thr210)Colorimetric Cell-Based ELISA Kit

A colorimetric cell-based ELISA kit from Assay Genie designed for the accurate detection and quantification of phosphorylated NEK9 at threonine 210 in cell lysates. The assay uses NEK9 (Phospho-Thr210)-specific primary antibodies to capture the target, with HRP-conjugated secondary antibodies binding the Fc region of the primary antibody; the conjugated HRP then catalyses a colorimetric reaction upon substrate addition, read at 450 nm. The kit is described as convenient, lysate-free, high throughput and sensitive, and operates in an indirect ELISA format suitable for measuring relative phosphorylated NEK9 levels in cultured cells as well as screening the effects of treatments, inhibitors (such as siRNA or chemicals) or activators on NEK9 phosphorylation.

Reactivity covers Human, Mouse and Rat samples, with a dynamic range of greater than 5000 cells. The workflow includes an anti-GAPDH monoclonal antibody as an internal positive control for normalizing target absorbance values, and a Crystal Violet whole-cell staining method may be applied after the colorimetric read to determine cell density and adjust for plating differences. NEK9 is described as a critical serine/threonine kinase involved in cell cycle regulation and centrosome duplication, with phosphorylation at threonine 210 linked to its activity, supporting research into cell cycle control, mitotic progression, and signalling pathways relevant to cancer and neurodegenerative disorders.