NCoR1 Transcription Factor Activity Assay

The NCoR1 (Nuclear Receptor Corepressor 1) Transcription Factor Activity Assay from Assay Genie is a microplate-based DNA-binding ELISA Kit for quantifying the DNA-binding activity of the NCoR1 corepressor in nuclear and cell lysates. The assay provides a non-radioactive alternative to traditional electrophoretic mobility shift assays (EMSA), enabling sensitive and high-throughput analysis of NCoR1 function in transcriptional regulation. NCoR1 is a key regulator that mediates ligand-independent transcriptional repression by nuclear receptors, including thyroid hormone receptor and retinoic acid receptor, and plays essential roles in metabolism, inflammation, circadian rhythm, and cancer signaling pathways. The kit is reactive across Human, Mouse, and Rat species, making it broadly useful for gene regulation research, hormone signaling studies, and investigations of corepressor-mediated chromatin remodeling. Reagents should be stored according to the package insert; aliquot reconstituted components to minimize freeze-thaw cycles and protect from light. For research use only; not intended for diagnostic applications.