NCBP2 Colorimetric Cell-Based ELISA

Designed for the accurate detection of NCBP2 in cultured cells, this Colorimetric Cell-Based ELISA Kit from Assay Genie offers a high-throughput, lysate-free workflow for profiling target protein expression directly in cells. The qualitative indirect ELISA format uses NCBP2-specific primary antibodies, with HRP-conjugated secondary antibodies that catalyze a colorimetric reaction read at 450 nm. The kit can be used to measure relative NCBP2 levels in cultured cells and to screen the effects of various treatments, inhibitors (such as siRNA or chemicals), or activators on NCBP2 expression.

For data normalization, a monoclonal antibody specific for human GAPDH is included as an internal positive control, and a Crystal Violet whole-cell staining step can be used to determine cell density, allowing absorbance values to be normalized to cell amounts. The assay has a stated dynamic range of > 5000 cells and is reactive with Human and Mouse samples. It is supplied in a 96-Well Microplate format and is stored at 4°C with a stated stability of 6 months.

NCBP2, also known as Nuclear cap-binding protein subunit 2, is a component of the nuclear cap-binding complex (CBC), which binds co-transcriptionally to the 5' cap of pre-mRNAs and is involved in pre-mRNA splicing, translation regulation, nonsense-mediated mRNA decay, RNA-mediated gene silencing by microRNAs, and mRNA export from the nucleus. Aberrant expression of NCBP2 has been linked to various diseases, including cancer, neurodevelopmental disorders, and viral infections, supporting its relevance as a research target.