NAB2 Colorimetric Cell-Based ELISA

From Assay Genie, this Cell-Based ELISA Kit provides a sensitive, lysate-free, high-throughput workflow for detecting NAB2 protein expression directly in cultured cells. The assay uses an indirect ELISA format in which NAB2-specific primary antibodies capture the target in fixed cells, and HRP-conjugated secondary antibodies then catalyze a colorimetric reaction upon substrate addition. Detection is performed colorimetrically at 450 nm, and qualitative results are normalized using an included anti-GAPDH monoclonal antibody as an internal positive control together with Crystal Violet whole-cell staining to account for differences in cell density.

Reactivity is reported for Human and Mouse samples, and the kit is intended for use with adherent cells. It supports measurement of relative NAB2 levels in cultured cells and screening of the effects of various treatments, inhibitors (such as siRNA or chemicals), or activators. Storage is at 4 °C with a stability of 6 months, and the workflow is configured around a 96-well microplate.

NAB2 (NGFI-A-binding protein 2) is a nuclear transcriptional repressor for the zinc finger transcription factors EGR1 and EGR2 and is implicated in cell proliferation, nervous system development, and negative regulation of transcription from RNA polymerase III promoters. The human ortholog maps to chromosomal location 12q13.3 and encodes a protein of 49,748 Da. The research area is Epigenetics and Nuclear Signaling, with relevance to studies of cancer and neurological disorders where NAB2 dysregulation has been reported.