MYT1 Colorimetric Cell-Based ELISA Kit

Assay Genie offers a colorimetric, cell-based ELISA for the qualitative measurement of MYT1 protein levels in human samples. The kit supports detection in serum, plasma, lysate samples, and cell culture supernatants, and is intended for use with adherent cells in a microplate format. It provides a lysate-free, high-throughput workflow with a simple protocol suited to screening the effects of treatments, inhibitors (such as siRNA or chemicals), and activators on MYT1 expression.

Detection follows an indirect ELISA format in which MYT1-specific primary antibodies capture the target and HRP-conjugated secondary antibodies bind the Fc region of the primary antibody. Substrate addition produces a colorimetric reaction read at 450 nm, with a stated dynamic range of > 5000 cells and storage specified at 4°C for 6 months. Two normalization methods are included: a monoclonal antibody specific for human GAPDH serves as an internal positive control for target absorbance, while Crystal Violet whole-cell staining is used to determine cell density and adjust for plating differences.

The target corresponds to PKMYT1 (UniProt Q99640, NCBI Gene ID 9088), a membrane-associated dual-specificity kinase of the WEE family with a molecular weight of 47,284 Da. According to the supplied information, this protein negatively regulates the G2/M transition of the cell cycle by phosphorylating and inactivating CDC2, placing the assay within cell cycle research applications.