MYC Transcription Factor Activity Assay

Quantitative measurement of MYC transcription factor DNA-binding activity in nuclear and whole-cell lysates prepared from human, mouse, and rat samples. This DNA-binding ELISA kit from Assay Genie uses a colorimetric readout and is supplied in a 96 Assays microplate format for active c-Myc studies in cancer biology, signal transduction, and gene regulation research.

MYC is a basic helix-loop-helix-leucine zipper transcription factor that recognizes E-box DNA motifs (CACGTG) and drives expression of genes controlling proliferation, metabolism, ribosomal biogenesis, and apoptosis. Aberrant MYC activity is implicated in a broad spectrum of human malignancies, making reliable quantification of its DNA-binding capacity a frequent requirement in oncology drug screening and mechanistic cell biology workflows.

Researchers apply the kit to nuclear extracts prepared from cultured cells or fresh tissue to monitor changes in MYC activation following genetic manipulation, small-molecule treatment, or growth factor stimulation. The assay tolerates standard lysis buffers used for transcription factor extraction and supports both small pilot studies and larger in vitro screening campaigns across multiple biological replicates, providing a quantitative index of transcription factor occupancy relevant to pathway analysis and target validation.