MYC Colorimetric Cell-Based ELISA Kit

A colorimetric cell-based ELISA kit from Assay Genie is designed to detect and quantify MYC protein levels directly in cultured cells. Described as a convenient, lysate-free and high-throughput assay, it uses an indirect ELISA format in which MYC-specific primary antibodies capture the target while HRP-conjugated secondary antibodies bind the Fc region of the primary antibody, enabling a colorimetric reaction upon substrate addition.

The kit is reactive with Human, Mouse and Rat samples and is supplied in a 96-Well Microplate format with a stated dynamic range of > 5000 Cells. Detection is read at 450 nm and the kit is intended to be stored at 4°C for 6 Months.

A monoclonal antibody specific for human GAPDH is included as an internal positive control for normalising target absorbance values, and a Crystal Violet whole-cell staining step may be used to determine cell density for further normalisation. The protocol is described for adherent cells, and the kit can be used to measure relative MYC levels as well as to screen the effects of treatments, inhibitors (such as siRNA or chemicals) or activators on MYC expression in cells.