MYB Transcription Factor Activity Assay

The MYB (c-Myb) transcription factor is a master regulator of hematopoietic stem cell self-renewal, proliferation, and differentiation, with dysregulated MYB activity implicated in leukemia, breast, and colorectal cancers. This DNA-binding ELISA from Assay Genie uses microplate wells pre-coated with a double-stranded oligonucleotide containing the MYB consensus response element to capture active MYB from nuclear and cell lysates, letting researchers quantify DNA-binding activity rather than just total protein levels. Bound MYB is detected with a sequence-specific primary antibody and an HRP-conjugated secondary, and the chromogenic signal is read on a standard plate reader. The 96 Assays format supports multiple replicate experiments across human, mouse, and rat samples. For best results, prepare nuclear extracts on ice, aliquot to limit freeze-thaw cycles, and refrigerate most kit components; store the working detector antibody frozen at -20 degrees C between runs. Intended for in vitro research use only, this kit is a useful tool for studies of transcriptional control in stem cell biology, oncology, and differentiation pathways.