MYB (Phospho-Ser12)Colorimetric Cell-Based ELISA Kit

This cell-based ELISA kit from Assay Genie is designed for the detection of MYB phosphorylation at serine 12 in adherent cell cultures. The kit uses a colorimetric detection method read at 450 nm and is supplied in a 2 x 96-well microplate format suited to high-throughput screening of phosphorylation dynamics in response to various treatments, inhibitors, or activators.

The assay employs an indirect ELISA format in which MYB (Phospho-Ser12) is captured by phospho-specific primary antibodies, while HRP-conjugated secondary antibodies bind the Fc region of the primary antibody to catalyze a colorimetric reaction upon substrate addition. A monoclonal anti-GAPDH antibody is included as an internal positive control for normalizing target absorbance values, and a Crystal Violet whole-cell staining step can be used to determine cell density. The kit has a stated dynamic range of > 5000 cells and is reactive with human and mouse samples.

Categorised within the Epigenetics and Nuclear Signaling research area, this phospho-specific kit is intended for studies investigating MYB signalling in processes such as cancer and hematopoiesis, supporting research into regulatory mechanisms of MYB downstream target gene expression.