mTOR (Phospho-Ser2448)Colorimetric Cell-Based ELISA Kit

This cell-based ELISA kit from Assay Genie is designed to study the phosphorylation status of mTOR at serine 2448 in cell lysates. Phosphorylation of mTOR at this site plays a role in the activation of mTOR signalling pathways, making the kit useful for research into cancer, diabetes and other diseases where mTOR is a key regulator of cell growth, metabolism and proliferation.

The kit enables detection of mTOR protein phosphorylation and expression profile in cultured cells, supporting measurement of relative phosphorylated mTOR levels and screening for the effects of treatments, inhibitors (such as siRNA or chemicals) or activators on mTOR phosphorylation. It uses an indirect ELISA format in which a primary antibody specific for mTOR (Phospho-Ser2448) captures the target, and an HRP-conjugated secondary antibody binds the Fc region of the primary antibody, catalysing a colorimetric reaction upon substrate addition. A monoclonal antibody against human GAPDH is included as an internal positive control for normalisation, and a Crystal Violet whole-cell staining method may be used to determine cell density so that absorbance values can be normalised to cell amounts.

The kit is reactive with Human, Mouse and Rat samples and is suited to adherent cells. Detection is colorimetric at 450 nm, with a dynamic range of > 5000 Cells. Target: mTOR (Phospho-Ser2448); Sub Type: Phospho; ELISA Type: Cell-Based. Database references: Gene ID 2475, UniProt ID P42345.