MRE11 Colorimetric Cell-Based ELISA Kit

Assay Genie offers a colorimetric cell-based ELISA kit for the sensitive detection of MRE11 in cell lysates and tissue homogenates. The qualitative assay uses an indirect ELISA format, in which MRE11 is captured by specific primary antibodies and detected via HRP-conjugated secondary antibodies that catalyze a colorimetric reaction at 450 nm upon substrate addition.

Reactive with human, mouse and rat samples and supporting a dynamic range of > 5000 cells, the kit is intended for measuring relative MRE11 protein expression in cultured cells and for screening the effects of treatments, inhibitors (such as siRNA or chemicals) or activators on MRE11. Because the assay is qualitative, two normalization strategies are provided: a monoclonal anti-GAPDH antibody serves as an internal positive control, and a Crystal Violet whole-cell staining step can be used to determine cell density for adjusting absorbance values.

MRE11A is a nuclear protein that functions in the DNA damage response, with roles in homologous recombination, telomere length maintenance and DNA double-strand break repair, and forms a complex with the RAD50 homolog. Dysregulation of MRE11 has been linked to cancer and neurodegenerative disorders, supporting its relevance as a potential biomarker and therapeutic target. The kit is stored at 4 °C with a stated stability of 6 months. Database identifiers: Gene ID 4361, UniProt ID P49959.