Mouse X-Ray Repair Cross Complementing 5 (XRCC5) ELISA Kit

Repair of DNA double-strand breaks through the canonical non-homologous end joining pathway depends on the Ku70/Ku80 heterodimer, with Ku80 itself encoded by X-Ray Repair Cross Complementing 5 (XRCC5). This colorimetric ELISA Kit from Assay Genie delivers quantitative measurement of mouse XRCC5/Ku80 protein, supporting laboratories interrogating genomic stability, radiobiology, genotoxicity, chemoresistance, and tumor cell responses to DNA-damaging agents.

Validated for mouse tissue homogenates and compatible specimen matrices, the kit supplies reagents sufficient for 96 assays in a standard microplate format. Researchers can profile Ku80 expression across treatment cohorts, compare knockout versus wild-type cohorts, or follow time-course responses after irradiation, oxidative insult, or chemical exposure in murine models. The protocol supports studies of V(D)J recombination, telomere maintenance, and the cellular response to genotoxic stress.

Each kit is intended for research use only. Store kit components refrigerated and equilibrate the microplate to room temperature before initiating the protocol.