Mouse WNT3A ELISA Kit

Assay Genie offers a sandwich ELISA, double antibody kit designed for the quantitative measurement of WNT3A in mouse-derived samples. WNT3A is described as a key signaling protein involved in pathways that regulate cell growth, differentiation, and development, with dysregulation linked to cancer, osteoporosis, and developmental disorders. The kit is positioned for metabolism research and the target protein is catalogued under UniProt P27467.

Validated for mouse reactivity, the assay measures WNT3A with a sensitivity of 0.188 ng/mL and a working range of 0.313-20 ng/mL. Accepted sample types include serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. Kits should be stored at 2-8°C for 12 months.

Assay performance has been demonstrated through linearity and recovery testing. Linearity results at dilutions of 1:2, 1:4 and 1:8 were: serum (n=5) 85-102%, 86-102%, 86-103%; EDTA plasma (n=5) 82-97%, 85-100%, 82-100%; heparin plasma (n=5) 82-95%, 81-90%, 86-96%. Recovery testing returned average recoveries of 95% for serum, 93% for EDTA plasma and 94% for heparin plasma.