Mouse Wingless Type MMTV Integration Site Family, Member 10B (WNT10B) ELISA Kit

The Assay Genie mouse WNT10B quantitative immunoassay kit enables researchers to measure wingless-type MMTV integration site family member 10B concentrations in mouse research specimens. WNT10B is a secreted glycoprotein ligand of the Wnt/β-catenin signaling pathway, studied extensively for its roles in osteoblast differentiation, adipogenesis regulation, dental development, and stem cell biology. Investigators apply this kit to tissue homogenate and serum samples harvested from mouse models of metabolic dysfunction, skeletal disorders, and hair follicle morphogenesis.

Supplied as a microplate-based assay package with pre-coated wells, biotinylated detection reagent, standard calibrators, and chromogenic substrate, the kit supports a complete immunoassay workflow. The protocol follows standard addition, incubation, washing, and enzymatic development steps readable on conventional microplate absorbance equipment. A standard curve generated from the kit's reference preparation allows interpolation of sample concentrations. The product is labeled for research use only.

WNT10B belongs to the canonical Wnt ligand family of secreted cysteine-rich glycoproteins. Its dysregulation has been documented in obesity research, bone density studies, and investigations of pilomatrixoma and other epithelial neoplasms. Storage guidance recommends keeping unopened kit components refrigerated until first use, with working dilutions of standards and detection reagents stored frozen at -20°C between experiments to maintain reagent activity.