Mouse VISTA/B7-H5/PD-1H PharmaGenie ELISA Kit (SBRS1565)

Built for immunology and immuno-oncology laboratories, this quantitative immunoassay from Assay Genie enables precise measurement of mouse VISTA (also known as B7-H5 or PD-1H) in plasma, serum, and cell culture supernatant samples. VISTA is a B7-family immune checkpoint receptor expressed predominantly on myeloid-lineage cells and certain T-cell subsets, where it regulates peripheral tolerance, T-cell exhaustion, and tumor immune evasion, making it a target of growing interest in checkpoint blockade research and combination immunotherapy studies.

The kit employs a matched antibody pair with an absorbance-based readout and is supplied in a microplate format containing reagents for 96 individual determinations. Plasma samples are typically collected using heparin or EDTA anticoagulants, while serum is prepared by standard clotting protocols; cell culture supernatant samples should be clarified by centrifugation before loading. Researchers studying autoimmune disease models, graft-versus-host disease, sepsis, or solid tumor microenvironments commonly use this kit to quantify soluble VISTA in mouse preclinical samples.

The kit components should be refrigerated upon arrival; for extended shelf life, individual reagents may be aliquoted and frozen according to the included protocol to avoid repeated freeze-thaw cycles of the detection antibody and standard. The reagents are intended for research use only and are not validated for diagnostic or clinical applications.