Mouse VEGF-D (Vascular Endothelial cell Growth Factor D) ELISA Kit (MOES01607)

Assay Genie supplies a sandwich-format ELISA for the quantitative detection of vascular endothelial growth factor D (VEGF-D) in mouse serum, plasma and cell culture supernatants. The colorimetric method also covers other biological fluids, with a stated sample volume of 100 μL, and the kit is listed for research in cancer, cardiovascular biology and signal transduction.

Reported performance figures include a detection range of 31.25-2000 pg/mL, sensitivity of 18.75 pg/mL and recovery of 80%-120%. In the recovery study, EDTA plasma gave a range of 90-106% with an average of 97%, and cell culture media gave 90-107% with an average of 98%. Intra-assay CV values were 6.98%, 6.57% and 6.43%, while inter-assay CV values were 8.54%, 8.33% and 7.63%. The manufacturer states that the kit recognizes mouse VEGF-D and that no significant cross-reactivity or interference with analogues was observed. Assay time is 3 h 30 min and storage is 2-8°C.

Components include a dismountable micro ELISA plate, reference standard, concentrated biotinylated detection antibody, concentrated HRP conjugate, reference standard and sample diluent, detection antibody diluent, HRP conjugate diluent, concentrated wash buffer, substrate reagent, stop solution, plate sealer, technical manual and certificate of analysis. VEGF-D is also listed under the synonyms FIGF, VEGFD and c-fos induced growth factor.