Mouse Uroporphyrinogen Decarboxylase (UROD) ELISA Kit

Researchers studying hepatic heme biosynthesis and porphyria can quantify mouse uroporphyrinogen decarboxylase (UROD) levels with this 96-assay immunoassay from Assay Genie. UROD catalyzes the fifth step of the heme synthesis pathway, sequentially decarboxylating uroporphyrinogen III to coproporphyrinogen III, and altered expression is implicated in Porphyria Cutanea Tarda, hepatoerythropoietic porphyria, and rodent models of chemical hepatotoxicity.

The kit is configured for use with mouse tissue homogenates and supports colorimetric measurement of enzyme abundance across experimental conditions. The pre-coated plate format enables batch processing of multiple biological replicates, with standards and detection reagents supplied in ready-to-use vials.

Typical applications include:

• Profiling hepatic injury responses in drug-treated mouse cohorts
• Evaluating iron-overload phenotypes and related metabolic stress
• Screening uroporphyria-relevant gene knockouts and CRISPR-edited lines
• Benchmarking heme-pathway flux across transgenic models
• Studying erythroid differentiation and erythropoietic regulation

All reagents are supplied for research use only; refer to the lot-specific protocol insert for storage temperature, reconstitution volumes, and recommended aliquot handling.