Mouse Tyrosine-protein kinase JAK3 (Jak3) ELISA Kit

Assay Genie offers this Sandwich ELISA Kit for the accurate quantification of Tyrosine-protein kinase JAK3 (Jak3), also known as Janus kinase 3 or JAK-3, in mouse samples. The kit employs a colorimetric ELISA detection method and is validated for use with serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.

JAK3 is a non-receptor tyrosine kinase predominantly expressed in immune cells, where it mediates essential signaling events in innate and adaptive immunity and plays a crucial role in hematopoiesis during T-cell development. Dysregulation of JAK3 has been linked to autoimmune disorders, inflammatory diseases, and certain types of leukemia, making it an important biomarker and potential therapeutic target. The kit shows specificity for natural and recombinant mouse Tyrosine-protein kinase JAK3 and is intended for research use only in signal transduction studies.

Performance characteristics reported for this kit include a detection range of 0.156-10ng/mL and a sensitivity of 0.09ng/mL. Intra-assay CV is 5.9% and inter-assay CV is 7.7%. Linearity in serum (N=5) ranges from 87-118% across 1:2 to 1:16 dilutions, with EDTA plasma (N=5) showing 93-109% and heparin plasma (N=5) showing 86-116%. Recovery is 97% (range 91-103) for serum and 99% (range 93-105) for plasma. The UniProt accession for the target is Q62137.