Mouse Tyrosine-protein kinase BTK (Btk) ELISA Kit

Designed for the quantitative measurement of mouse Bruton's Tyrosine Kinase, this Sandwich ELISA Kit from Assay Genie delivers a colorimetric detection workflow tailored to immunology research. Reactive with mouse samples, it accepts serum, plasma, tissue homogenates, cell culture supernatants and other biological fluids, and detects both natural and recombinant mouse Tyrosine-protein kinase BTK. Synonyms associated with the target include Agammaglobulinemia tyrosine kinase, B-cell progenitor kinase, Bruton tyrosine kinase, Kinase EMB, ATK, BPK and Bpk.

Reported performance characteristics include a detection range of 0.156-10 ng/mL and a sensitivity of 0.092 ng/mL, with an intra-assay CV of 8.8% and an inter-assay CV of 11.0%. Linearity in serum (N=5) spans 87-97% at 1:2 dilution through 93-103% at 1:16 dilution; EDTA plasma gives 95-106% at 1:2 falling to 94-103% at 1:16, while heparin plasma yields 103-116% at 1:2 falling to 103-113% at 1:16. Recovery averages 97% (range 91-103%) for serum and 99% (range 93-105%) for plasma.

BTK is described as a non-receptor tyrosine kinase indispensable for B lymphocyte development, differentiation and signaling, and has been linked to autoimmune disease, B-cell malignancies and immunodeficiencies. The UniProt reference is P35991. The kit is intended for research use only; component-specific storage conditions are detailed in the product manual.