Mouse Tumor necrosis factor receptor superfamily member 6 (Fas) ELISA Kit

Manufactured by Assay Genie, this Sandwich ELISA Kit enables quantitative measurement of mouse Tumor necrosis factor receptor superfamily member 6 (Fas), also referred to as CD95. The colorimetric assay is validated for use with mouse serum, plasma, tissue homogenates, cell culture supernates and other biological fluids. Reported performance specifications include a detection range of 78-5000pg/mL, a sensitivity of 39.5pg/mL, an intra-assay CV of 6.3% and an inter-assay CV of 7.8%. Linearity has been assessed in serum, EDTA plasma and heparin plasma across serial dilutions from 1:2 to 1:16, with reported recovery of 96% (range 90-102%) in serum and 98% (range 92-104%) in plasma.

Fas functions as a receptor for TNFSF6/FASLG, with the adapter molecule FADD recruiting caspase-8 to the activated receptor. The resulting death-inducing signaling complex initiates the caspase cascade that mediates apoptosis, with additional roles described in the induction of peripheral tolerance and in the antigen-stimulated suicide of mature T-cells. The kit is positioned as a tool for researchers studying apoptosis, immune response regulation and related disease models in mouse systems, with dysregulation of FAS signaling implicated in cancer, autoimmune disorders and inflammatory conditions. The product is supplied for research use only.