Mouse Tumor necrosis factor receptor superfamily member 1B (Tnfrsf1b) ELISA Kit

From Assay Genie, this Sandwich ELISA Kit provides quantitative detection of mouse tumor necrosis factor receptor superfamily member 1B (Tnfrsf1b), also known by the synonyms Tumor necrosis factor receptor 2, Tumor necrosis factor receptor type II, p75, p80 TNF-alpha receptor, TNF-R2, TNF-RII, CD120b, Tnfr-2 and Tnfr2, and is reactive with mouse samples (S1).

The kit detects natural and recombinant mouse Tnfrsf1b and is validated for serum, plasma, tissue homogenates, cell culture supernates and other biological fluids (S1). Catalogue attributes from Assay Genie list the detection method as colorimetric with sample types of serum and plasma (C0); the manufacturer datasheet describes the detection method as ELISA with the broader sample panel noted above (S1). The format is solid phase (S2). UniProt-derived information included with the kit states that Tnfrsf1b is a receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha, with TRAF1/TRAF2 recruiting the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2 (S1).

Manufacturer-reported performance figures include a detection range of 15.6-1000 pg/ml, a sensitivity of 7.83 pg/mL, an intra-assay CV of 6.4% and an inter-assay CV of 8.9% (S1). Recovery is given as 80-88% (mean 82%) for serum and 80-90% (mean 84%) for plasma (S1). Storage is at -20°C with shipping at +4°C (S2). For research use only (S1).