Mouse Tumor Necrosis Factor Beta (TNFb) ELISA Kit

The Mouse Tumor Necrosis Factor Beta (TNFb) ELISA Kit from Assay Genie enables quantitative measurement of lymphotoxin-alpha (LT-α), a cytokine secreted primarily by lymphocytes that contributes to lymphoid organ development, inflammation signaling, and tumor biology research. The assay uses a sandwich immunoassay performed on a 96-well microplate pre-coated with a capture antibody specific for murine TNFb.

Reported assay specifications include a detection range of 15.6 to 1000 pg/mL, a sensitivity below 9.4 pg/mL, and a sample volume of 100 uL per well. Reconstituted standards and detection reagents should be stored frozen at -20 degrees C in single-use aliquots to limit freeze-thaw cycles, while the sealed microplate is kept refrigerated at 4 degrees C. Reconstitution, washing, and incubation steps follow the protocol insert; absorbance is read on a standard microplate reader for colorimetric quantitation against a seven-point standard curve.

This Assay Genie kit is intended for research use only and supports studies in cytokine profiling, inflammatory disease models, and immune regulation in mouse systems. Compatible matrices include serum, plasma, tissue homogenates, cell lysates, and cell culture supernatants, making it applicable across in vitro and in vivo experimental workflows in immunology and inflammation research.