Mouse Tubulin Polymerization Promoting ProteinTPPP/TPPP ELISA Kit

From Assay Genie, this sandwich ELISA kit enables quantitative detection of Tubulin Polymerization Promoting Protein (TPPP) in mouse samples using a colorimetric, double-antibody format. The Mouse TPPP ELISA Kit is intended for research applications including studies of tubulin polymerization, a process essential for cell division, migration, and intracellular transport. Dysregulation of TPPP has been associated with neurodegenerative diseases such as Parkinson's and Alzheimer's, highlighting its relevance as a potential therapeutic target.

The kit is designed for use with serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. It offers a sensitivity of 0.188 ng/mL and a working range of 0.313–20 ng/mL. Components are stored at 2–8°C, with the kit remaining stable for 12 months under these conditions.

The assay procedure involves equilibrating reagents and TMB substrate to room temperature, primary incubation at 37°C for 90 minutes, biotin-labeled detection antibody binding, HRP-Streptavidin (SABC) binding, color development with TMB substrate in the dark for 10–20 minutes, and measurement of absorbance at 450 nm immediately after adding stop solution.