Mouse Tryptophan Hydroxylase 2 (TPH2) ELISA Kit

TPH2 is the brain-enriched tryptophan hydroxylase isoform that catalyzes the rate-limiting step in neuronal serotonin (5-HT) biosynthesis, expressed predominantly in the dorsal and median raphe nuclei. Quantifying TPH2 protein levels in mouse models supports research into serotonergic signaling, mood regulation, neurodevelopment, and stress-related behavioral phenotypes.

This ELISA kit from Assay Genie is configured for mouse-derived samples and validated for tissue homogenates and cell lysates, accommodating brain tissue preparations from dissected raphe regions, hippocampus, cortex, or whole-brain extracts. The assay produces an absorbance-based readout compatible with standard microplate readers and is supplied as a complete research-use-only reagent set formatted for 96 determinations per kit.

Applications include studies of knockout and transgenic mouse lines, chronic stress paradigms, and pharmacological interventions targeting serotonergic pathways, providing protein-level confirmation that complements transcript and enzyme-activity data. Researchers in neuroscience, neuropsychopharmacology, and behavioral biology use this TPH2 ELISA to measure central serotonin synthesis capacity where TPH2 regulation is a primary experimental variable, including investigations of depression-like behavior, anxiety models, and antidepressant drug response.