Mouse Tryptase beta-2 (Tpsb2) ELISA Kit

Assay Genie supplies a sandwich ELISA kit for the quantitative detection of mouse Tryptase beta-2 (Tpsb2), a target also referenced by the synonyms Mast cell protease 6, mMCP-6, Tryptase-2, and Mcpt6. Intended for research use only, the assay is validated for use with mouse serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

Quoted performance characteristics include a detection range of 0.156-10 ng/mL, a sensitivity of 0.068 ng/mL, an intra-assay CV of 4.9%, and an inter-assay CV of 8.1%. The kit is specific for natural and recombinant mouse Tryptase beta-2. Linearity was assessed in serum, EDTA plasma, and heparin plasma across serial dilutions, and recovery averaged 89% in serum (range 83-95%) and 91% in plasma (range 85-97%).

Tryptase beta-2 is described in the product documentation as a major neutral protease of mast cells that is secreted upon the coupled activation-degranulation response and plays a role in innate immunity, including an immunoprotective role during bacterial infection such as K. pneumoniae. The protein is listed under UniProt P21845 with a reported molecular weight of 25,829 Da, and is of research interest in cell biology, immune responses, inflammation, asthma, and allergic reactions.