Mouse Tripartite motif-containing protein 3 (Trim3) ELISA Kit

Assay Genie offers a sandwich ELISA for the quantitative detection of mouse Tripartite motif-containing protein 3 (Trim3) in mouse samples. Trim3 is also referred to by the synonyms RING finger protein 22, RING finger protein HAC1, HAC1, and RNF22. According to the manufacturer, the protein is probably involved in vesicular trafficking via its association with the CART complex and positively regulates motility of the microtubule-dependent motor protein KIF21B.

The kit is for research use only and detects natural and recombinant mouse Tripartite motif-containing protein 3. Quoted performance characteristics include a detection range of 0.312-20ng/mL, a sensitivity of 0.172ng/mL, an intra-assay CV of 6.0%, and an inter-assay CV of 8.2%. Validated sample types include serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids. Reported serum recovery averages 86% with a range of 80-92%, and plasma recovery averages 88% with a range of 82-94%.

The target is reported to localize to the cytoplasm, early endosome, Golgi apparatus, trans-Golgi network, and cell projections including dendrites. Associated identifiers listed by the manufacturer include UniProt Q9R1R2, NCBI Gene ID 55992, and a molecular weight of 81kDa.