Mouse Trefoil Factor 3, Intestinal (TFF3) ELISA Kit

Trefoil Factor 3 (TFF3) is a small secreted peptide belonging to the trefoil factor family, named for its characteristic three-loop trefoil domain. Produced primarily by goblet cells of the intestinal mucosa, TFF3 contributes to epithelial restitution, mucin stabilization, and barrier protection following gastrointestinal injury.

Assay Genie's Mouse Trefoil Factor 3 (TFF3) ELISA Kit provides a quantitative immunological tool for profiling this mucosal marker across mouse-derived specimens. The kit supports research into colitis models, inflammatory bowel disease pathophysiology, gastric ulcer healing kinetics, and gut microbiome interactions, where TFF3 serves as a biomarker of mucosal recovery and goblet cell activity.

Validated starting materials include tissue homogenates, cell lysates, and culture supernatants from in vitro experiments. Reagents are supplied as a complete kit format with components sufficient for 96 individual assays, including a pre-coated microplate, detection antibody, standards, buffers, and substrate.

Intended for research use only, the assay produces a measurable signal proportional to TFF3 concentration in each specimen. Reagent stability is maintained by storing components according to the package insert, with reconstituted materials handled to limit repeated freeze-thaw events.