Mouse Transmembrane protease serine 4 (Tmprss4) ELISA Kit

From Assay Genie, this is a sandwich ELISA kit intended for the detection of mouse Transmembrane Protease, Serine 4 (TMPRSS4) levels. The kit is designed for use with mouse serum, plasma, tissue homogenates, cell culture supernatants and other biological fluids, and shows specificity for natural and recombinant mouse TMPRSS4. Synonyms associated with the target include Channel-activating protease 2, mCAP2 and CAP2.

Performance characteristics reported on the manufacturer's datasheet include a detection range of 1.56-100ng/mL and a sensitivity of 0.786ng/mL. Reproducibility is described as an intra-assay CV of 4.4% and an inter-assay CV of 8.3%. Linearity has been evaluated across serial dilutions in serum, EDTA plasma and heparin plasma matrices, and recovery values given are an average of 101% (range 95-107) for serum and 103% (range 97-109) for plasma. The kit is supplied for research use only and is categorised under the Cell Biology research area. The target protein is described as a probable protease that appears capable of activating ENaC, and is associated with the UniProt accession Q8VCA5.

Detection follows an ELISA workflow using the supplied detection reagents, wash buffer, substrate and stop solution, with absorbance read at 450 nm on a microplate reader. Component-specific storage conditions are detailed in the kit documentation.