Mouse Transforming Growth Factor Beta Receptor I (TGFbR1) ELISA Kit

From Assay Genie, this colorimetric ELISA Kit quantifies mouse Transforming Growth Factor Beta Receptor I (TGFbR1), also referred to as ALK5, a transmembrane serine/threonine kinase that drives canonical SMAD2/SMAD3 signaling downstream of TGF-beta ligands. The kit is configured for 96 assays and validated specifically for mouse reactivity, fitting into studies of fibrosis, epithelial-mesenchymal transition, immune regulation, and tumor cell plasticity. Compatible specimen types include tissue homogenates and cell lysates prepared from mouse sources, enabling researchers to track receptor abundance across treatment conditions, gene knockout models, and disease-state comparisons. The colorimetric readout integrates with standard microplate readers, requiring no specialized instrumentation beyond the absorbance measurement capabilities typically found in cell signaling laboratories. Each pack supplies reagents for 96 individual determinations, which is practical for medium-throughput screens of siRNA or CRISPR libraries, small compound libraries, and time-course experiments examining TGFbR1 modulation. Investigators working on receptor turnover, ligand-induced downregulation, or pathway activity in mouse models of pulmonary, hepatic, or renal fibrosis can apply this reagent to measure endogenous receptor levels in cultured cells and tissue-derived preparations, supporting both in vitro and in vivo research applications.