Mouse Transforming Growth Factor Beta 1 (TGFb1) ELISA Kit

From Assay Genie, this ELISA Kit is configured as 96 individual determinations and targets TGFb1, a 25 kDa homodimeric cytokine central to fibrosis, immune regulation, and extracellular matrix biology in mouse models. Researchers rely on it to quantify cytokine release across in vivo and in vitro systems, including platelet-poor plasma, tissue homogenates, and cell culture supernatants harvested from transgenic, knockout, or wild-type mouse strains.

Workflow begins with pre-coated wells to which standards and unknowns are added; bound analyte is then detected through a chromogenic substrate read by absorbance on standard microplate instrumentation. All buffers, standards, and detection reagents are packaged together as a complete kit, supporting routine work in core facilities or individual laboratories investigating regulatory T-cell function, wound healing, inflammation, or epithelial-mesenchymal transition.

Intended for research use only, the kit supports studies aiming to connect TGFb1 levels with downstream readouts such as SMAD phosphorylation, collagen deposition, or immunosuppression in tumor microenvironments. Running samples in duplicate and comparing against the supplied standard curve enables accurate interpolation across the working range, while freezing working aliquots of standards helps preserve activity across multiple experimental batches.