Mouse Transcription factor GATA-5 (Gata5) ELISA Kit

From Assay Genie, this microplate immunoassay enables quantitative measurement of mouse GATA-5 protein across research sample matrices. GATA-5 is a zinc-finger transcription factor implicated in cardiac morphogenesis, intestinal epithelial differentiation, and gonadal development, supporting investigations in developmental biology, cardiovascular research, and tissue-specific gene regulation.

The kit supplies all reagents needed for 96 determinations per run, including pre-coated wells, matched antibody pairs, recombinant protein standards, enzyme conjugate working solution, chromogenic substrate, and wash buffer concentrate. Researchers can analyze circulating blood fractions, cell lysates, and tissue homogenate preparations using the absorbance-based workflow, with endpoint results captured on a standard microplate reader.

Store kit components at 4°C to maintain reagent activity; freeze reconstituted standard aliquots at -20°C for long-term storage. The protocol uses a 50 µL sample volume per well and proceeds through incubation, washing, and signal-development steps over approximately 3.5 hours before stop solution addition and absorbance measurement.