Mouse Transcription factor E2-alpha (Tcf3) ELISA Kit

Quantitative measurement of mouse Transcription factor E2-alpha (Tcf3) is made straightforward with this complete ELISA workflow from Assay Genie. The kit targets the E2A gene product, a basic helix-loop-helix (bHLH) transcription factor essential for B-cell lineage commitment, V(D)J recombination, and lymphocyte differentiation, supporting research into hematopoietic development, immunoglobulin enhancer activity, and Wnt-beta-catenin pathway regulation, where Tcf3 acts as a context-dependent transcriptional repressor.

Validated for mouse reactivity, the assay accommodates tissue homogenates, cell lysates, and clarified supernatant fractions as input material. Each kit is supplied as a self-contained reagent set with a pre-coated plate, biotinylated detection antibody, recombinant standard, streptavidin-enzyme conjugate, and substrate solution. Researchers typically dispense 100 uL of prepared sample per well and incubate at room temperature before signal development. Reconstituted standard should be aliquoted to limit freeze-thaw events, and unopened kit components are shipped on cold packs and held refrigerated until first use.

The Tcf3 ELISA Kit from Assay Genie fits into workflows that include E-protein dimerization studies, Notch1 cross-talk analysis in T-cell development, and investigations of transcriptional dysregulation in B-cell acute lymphoblastic leukemia, where Tcf3-PBX1 fusion events drive oncogenic programs. Specificity for the murine E2A isoforms E12 and E47 makes the kit relevant for validating knockout phenotypes, siRNA knockdowns, and transgenic rescue experiments in mouse model systems.