Mouse tPA (Plasminogen Activator, Tissue) ELISA Kit (MOES01368)

Quantitative measurement of tissue plasminogen activator (tPA) in mouse research samples is supported by this immunoassay kit from Assay Genie. tPA is a serine protease central to the fibrinolytic cascade, where it catalyzes the conversion of plasminogen to plasmin, initiating fibrin clot breakdown. As a result, tPA quantification informs research areas including vascular biology, cerebral ischemia modeling, wound healing studies, and characterization of transgenic or knockout mouse lines with altered hemostasis. The kit format includes a pre-coated microplate, lyophilized standards, biotinylated detection antibody, streptavidin-HRP conjugate, wash buffer, substrate solution, and stop reagent sufficient for the stated number of determinations. Sample handling typically involves collection into citrate or EDTA anticoagulant tubes for plasma, or standard serum tubes, followed by centrifugation and storage at the indicated storage temperature prior to assay. Researchers process samples alongside a standard curve to interpolate tPA concentrations, with results generally expressed in ng/mL based on the recombinant mouse tPA reference standard included in the kit. All components are intended for research use only and should be stored as indicated on the packaging to preserve antibody binding activity and enzyme conjugate integrity. The complete kit format provides reproducibility for studies requiring quantitative comparison across treatment groups, time points, or genetic backgrounds.