Mouse Topoisomerase I (TOP1) ELISA Kit

Quantitative measurement of mouse topoisomerase I (TOP1) is achieved with this colorimetric immunoassay from Assay Genie, providing a 96-test format for in vitro research on DNA topology, replication stress, and chromatin remodeling in murine models. The kit detects TOP1 in tissue homogenates and cell lysates prepared from mouse specimens, supporting studies of enzyme regulation in cell culture and dissected tissue samples. Researchers in cancer biology, neurobiology, and molecular pharmacology use this assay to quantify topoisomerase I levels when investigating camptothecin sensitivity, replication-associated DNA damage, and transcriptional control.

Each kit contains a microwell plate, lyophilized standards, biotinylated detection antibody, and streptavidin-HRP reagents, with chromogenic readout quantified on a standard microplate reader at the appropriate wavelength. Sample wells are loaded alongside a standard curve, and absorbance values are converted to TOP1 concentrations. Reagents are supplied refrigerated and should be equilibrated to room temperature before initiating the protocol; the kit is intended for research use only and is not validated for diagnostic or therapeutic procedures.