Mouse Toll Like Receptor 4 (TLR4) ELISA Kit

Quantitative measurement of mouse Toll-like Receptor 4 in tissue homogenates, cell lysates, and cell culture supernatants is supported by this research-use immunoassay from Assay Genie. TLR4 acts as a transmembrane pattern recognition receptor that detects lipopolysaccharide from Gram-negative bacteria and triggers MyD88-dependent signaling cascades leading to NF-κB activation and pro-inflammatory cytokine release. Researchers studying sepsis, endotoxin tolerance, innate immune priming, or chronic inflammatory disease models in murine systems can use this kit to track TLR4 expression changes in macrophages, dendritic cells, epithelial tissue, or cultured cell lines. The assay accepts crude biological matrices including solubilized tissue, clarified lysates, and conditioned media without extensive pre-processing. Each kit is supplied with pre-coated plate strips, detection antibody, standard diluent, and substrate components needed to run quantitative standard curves. Reconstituted reagents and unused strips should be stored according to the accompanying package insert to preserve binding activity across repeated thaw cycles. Results support downstream applications including western blot confirmation of TLR4 protein, qPCR measurement of TLR4 transcript, and flow cytometry comparison of surface expression in matched samples.