Mouse Toll Like Receptor 3 (TLR3) ELISA Kit

The Mouse Toll Like Receptor 3 (TLR3) ELISA Kit from Assay Genie supports quantitative measurement of TLR3, an endosomal pattern recognition receptor central to antiviral innate immunity and dsRNA recognition. Investigators apply this kit in studies of viral pathogenesis, type I interferon signaling, dendritic cell maturation, and neuroinflammatory disease models using murine samples. The format uses a 96-well microplate and is validated against tissue homogenates, cell lysates, and cell culture supernatants, broadening its utility across immunology, virology, and inflammation research workflows.

Capture and detection antibodies are raised against murine TLR3 and demonstrate no significant cross-reactivity with other Toll-like receptor family members, enabling specific quantification of TLR3 expression changes in response to poly(I:C) stimulation or viral challenge. Each kit supplies 96 assays with all reagents needed to run the protocol, and the colorimetric endpoint is read at 450 nm on standard microplate readers.

Store unopened components refrigerated at 2-8°C and protect the chromogen and conjugate vials from light. Reconstituted standards and concentrated detection antibody should be aliquoted to limit freeze-thaw cycles, preserving binding activity across multiple experimental runs.