Mouse TNF-alpha PharmaGenie ELISA Kit (SBRS1541)

Assay Genie's Mouse TNF-alpha PharmaGenie ELISA Kit quantifies tumor necrosis factor alpha, a central pro-inflammatory cytokine released primarily by activated macrophages. The kit supports research into inflammatory disease, sepsis, autoimmune models, and host-pathogen immunity, where TNF-alpha elevation is a key biomarker. All reagents are supplied for immediate use in a microplate format, with standards, detection antibody, detection buffer, and wash solutions included for one plate. Using a sample volume of 100 uL per well, researchers can apply undiluted or appropriately diluted biological samples from cell culture, plasma, or serum sources to generate a dose-response curve. The capture antibody is specific to mouse TNF-alpha and exhibits minimal cross-reactivity with related cytokines such as IL-1 and IL-6. Reconstituted standards should be stored frozen in aliquots to limit freeze-thaw cycles and preserve immunoreactivity.