Mouse TNF-alpha PharmaGenie ELISA Kit (For Lysates) (SBRS1542)

Built for cytokine quantification directly in cellular and tissue lysates, this Assay Genie immunoassay enables researchers to measure mouse TNF-alpha, a 17 kDa pro-inflammatory cytokine central to macrophage-driven inflammation, acute phase responses, and NF-κB signaling studies. The kit is formatted for 96 assays using an absorbance-based readout, calibrated to the native murine protein so investigators can track TNF-alpha expression at the tissue level rather than relying on serum surrogates.

Lysate workflows benefit from compatibility with standard RIPA or NP-40 extraction protocols containing protease inhibitor cocktails; cleared supernatants are typically normalized to total protein before loading, and the assay tolerates the detergent concentrations common to these buffers. Each kit contains a pre-coated microplate, lyophilized recombinant standard, biotinylated detection antibody, streptavidin-enzyme conjugate, wash buffer, and chromogenic substrate, with all components intended for research use only and stable when stored refrigerated between uses.

Typical applications include profiling inflammatory responses in macrophage cell lines such as RAW 264.7, evaluating TNF-alpha induction in mouse disease models challenged with LPS, in colitis or arthritis systems, and comparing cytokine output across genetically modified mouse strains.